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高乌甲素对手术致痛大鼠脊髓Fos蛋白的影响
Effect of lappaconitione on Fos-protein of spinal cord in incision-induced rats

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DOI:
作者:
林密迦 林财珠 杨锡馨
LIN Mi-jia，LIN Cai-zhu，YANG Xi-xin
作者单位:
福建医科大学附属第一医院麻醉科
Department of Anesthesia,The Affiliated First Hospital,Fujian Medical University
关键词:
高乌甲素；大鼠切口疼痛模型；脊髓；Fos蛋白；免疫组化
lappaconitione; rat incision model; spinal cord; Fos-protein; immunity histochemistry
摘要:
【摘要】目的：研究高乌甲素对手术致痛大鼠脊髓Fos蛋白的影响。方法：40只大鼠随机分5组，每组各8只，均按Brennan法手术，建立大鼠手术切口疼痛模型：对照组（SaS组）术前16 min腹腔注射生理盐水；不同剂量术前给药组（L1S组、L2S组、L3S组）术前16 min分别腹腔注射高乌甲素1、2和4 mg/kg；术后给药组（SL3组）术后16 min腹腔注射高乌甲素4 mg/kg。1.5h后将大鼠灌注，取材和冰冻切片，SP法进行免疫组化染色，观察脊髓背角Fos免疫反应阳性神经元（FLIN）在脊髓上的变化情况。结果：不同剂量术前给药组间较对照组脊髓背角FLIN呈剂量依赖性减少(P＜0.05)；相同剂量高乌甲素术前给药组抑制Fos表达强于术后给药组(P＜0.01)；结论：高乌甲素腹腔注射可抑制手术致痛大鼠的脊髓背角fos表达，并呈量效关系；预先给药较术后给药镇痛效果好。
ABSTRACT: Objective To investigate effect of lappaconitione on Fos-protein of spinal cord in incision-induced rats. Merhods 40 awake rats were randomly invided into five groups,build the rat incision model:Control group(received lappaconitione saline vehicle ip 16 min prior to the incisions),differrent dose administered prior to the incisions(received lappaconitione 1,2,4 mg/kg ip respectively 16 min prior to the incisions),administered post to the incisions(received lappaconitione 4 mg/kg ip 16 min after the incisions).1.5h later,rats were killed and perfused ,L4-S1 sections of spinal cords were dissected,sliced by freezing. And then processed by immunostained with a rabbit polyclonal antiserum directed against c-fos and SP, then the changes of FLIN in the dorsal horn were studied. Results Lappaconitione treatment markedly decreased the number of FLIN in the dorsal horn in a dose-dependent manner. The analgesic effect of lappaconitione preincision is superior to lappaconitione postincision in Fos-protein suppression. Conclusion Fos-protein of spinal cord in dorsal horn were suppressed by lappaconitione administration in dose-dependent manner. The analgesic effect of lappaconitione given before pain stimulation was better than that given after incision.

 

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