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上海市卫生健康委员会
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米诺环素对大鼠脑缺血再灌注后细胞凋亡的抑制作用
The Inhibitory Effect of Minocycline on Apoptosis in Rats with Cerebral Ischemia –Reperfusion

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DOI:
作者:
熊建忠
XIONG Jian-zhong
作者单位:
萍乡市人民医院
The people’s Hospital of Pingxiang Jiangxi
关键词:
脑缺血再灌注；米诺环素；凋亡
Cerebral Ischemia-Reperfusion; Minocycline; Apoptosis
摘要:
摘要 目的 观察米诺环素对大鼠脑缺血再灌注后细胞凋亡的影响。方法 30只雄性SD大鼠随机分为假手术组、生理盐水组、预处理组、治疗组、小剂量组，每组6只。采用线栓法制作大鼠脑缺血再灌注模型。用RT-PCR、免疫组化、Hoechst染色、核磁共T2WI成像等方法，了解米诺环素对缺血后细胞凋亡的影响。结果 脑缺血后caspase-3、ICE表达增强，半暗带区凋亡细胞增多，T2WI扫描在缺血侧可见大片异常信号区。预处理组、治疗组、小剂量组caspase-3、ICE表达减少，细胞凋亡百分率下降，脑缺血体积缩小。结论 米诺环素能抑制缺血后细胞凋亡。
Abstract: Objective To research the effect of minocycline on the neuronal apoptosis in rats with cerebral ischemia-reperfusion injury. Methods Thirty healthy male Sprague-Dawley rats were randomly divided into, sham-operation group, control group, minocycline precondition-ing group, minocycline treatment group, low dose minocyclie group. Each group was 6. Middle cerebral artery occlusion in rats was established with the suture method. To study the inhibitory effect of minocycline on apoptosis in rats with cerebral ischemia-reperfusion injury with immunohistochemisry , reverse transcription polymerase chain reaction (RT-PCR), Hoechst stain and T2WI. Results The expression of caspase-3 and the interleukin-1β converting enzyme increased significantly in control group. The rate of apoptotic cells also increased significantly in control group. There were large abnormal signal areas in control group when were visualized with with T2-weighted. The relative infarction volumes decreased, the expression of the interleukin-1β converting enzyme and caspase-3 also decreased in minocycline precondition-ing group, minocycline treatment group, low dose minocyclie group. The rate of apoptotic cells in minocycline precondition-ing group, minocycline treatment group, low dose minocyclie group was significant lower than that of in control group. Conclusion The minocycline could inhibit the neuronal apoptosis in rats with cerebral ischemia-reperfusion.

 

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