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当前位置：首页 > 过刊浏览->2010年第11期

凋亡抑制基因Survivin和P63基因在喉癌组织中的表达及意义
Expression and clinical significance of survivin and p63 gene in laryngeal squamous cell carcinoma

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DOI:
作者:
刘学军,刘国钧,凡启军,陈波蓓,黄赛瑜,黄加云
作者单位:
温州医学院附属第二医院育英儿童医院
关键词:
Survivin；喉鳞状细胞癌；p63； 免疫组织化学
Survivin; LSCC; p63; immunohistochemistry
摘要:
【摘要】 目的 探讨凋亡抑制基因Survivin和P63基因在喉鳞状细胞癌（LSCC）组织中的表达及其与喉鳞癌发生发展的关系。方法 收集喉鳞状细胞癌标本42例，其中临床Ⅰ期5例(T1N0M0 5例)；临床Ⅱ期8例(T2N0M0 8例)；临床Ⅲ期18例(T3N0M0 8例， T1N1M0 2例，T2N1M0 4例，T3N1M0 4例)；临床Ⅳ期11例(T4N1M0 2例， T2N2M0 4例，T3N2M0 3例，T4N2M0 2例)。42例喉癌均取癌旁组织进行检测，另外取12例正常喉部组织进行对照，采用免疫组织化学链霉菌抗生物素蛋白-过氧化酶(S-P)法检测三种喉部组织中Survivin基因和P63基因的表达。结果 喉鳞状细胞癌中Survivin表达阳性率为66.7%（28/42）。临床分期Ⅲ+Ⅳ期Survivin阳性表达率显著高于Ⅰ+Ⅱ期；有淋巴结转移者的Survivin阳性表达率高于无淋巴结转移者，中低分化者Survivin阳性率显著高于高分化者（均p<0.05）；p63 蛋白在LSCC 组织、癌旁组织中的阳性表达率分别为90.5%(38/42)、85.7%(36/42) ,与正常喉组织50%(6/12)比较,差异均有统计学意义(P< 0. 05)；p63蛋白与喉癌的各临床病理特征无关。结论 Survivin基因与喉癌的发生、发展密切相关，有望成为喉癌诊断和基因治疗的新靶点，其高表达提示肿瘤患者预后不良；而p63蛋白对喉癌的生物学行为的影响相对较小。
【Abstract 】 Objective To explore the expression of Survivin and P63 in LSCC as well as Their correlations with the development of LSCC. Methods collect 42 samples of LSCC, among which 5 patients are at stage Ⅰ(5 T1N0M0 cases),8 at stage Ⅱ(8 T2N0M0 cases ),18 at stage Ⅲ(8 T3N0M0 cases,2 T1N1M0 cases，4 T2N1M0 cases，4 T3N1M0 cases), 11 at stage Ⅳ(2 T4N1M0 cases，4 T2N2M0 cases，3 T3N2M0 cases，2 T4N2M0 cases). We choose the 42 samples of LSCC and test the tissue nearby ,meanwhile choose 12 normal samples to be contrast, adoping S-P Method to detect the expression of Survivin and P63 gene in larynx tissue. Result The positive rate of Survivin expression in LSCC is 66.7% (28/42), the positive rate of Survivin expression at Clinical stage Ⅲ+Ⅳ is much higher than that at stageⅠ+Ⅱ, while the positive rate of Survivin in absorbent gland transferring cases is higher than those who haven’t；the positive rate of Survivin of those moderately-differentiated and poorly-differentiated is much higher than those well-differentiated (p<0.05) .The proteic expression rate of P63 in LSCC and the tissue nearby is 90.5%(38/42)、85.7%(36/42) respectively.Comparing with the normal larynx tissue 50%(6/12), the difference is notable (p< 0. 05); the P63 proteinum has nothing to do with diverse clinical patho-characters. Conclusion The expression of Survivin is closely correlated with the onset and development of LSCC and can be a new target to diagose and to perform gene therapy in LSCC. The high expression rate of Survivin tells poor unfavourable prognosis of patients; while the P63 proteinum has less influence on LSCC biological behaviour.

 

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